Inter-laboratory evaluation of three flagellin PCR/RFLP methods for typing Campylobacter jejuni and C. coli: the CAMPYNET experience
نویسندگان
چکیده
منابع مشابه
Molecular typing of Campylobacter jejuni and Campylobacter coli.
Shi et al. (7) presented data on the development and application of a PCR-based restriction fragment length polymorphism analysis of a 9.6-kb portion of the lipopolysaccharide gene (LG) cluster of Campylobacter jejuni and Campylobacter coli. This proposed molecular subtyping system is novel and has the potential of being directly applicable to epidemiology, prevalence, and other studies. I agre...
متن کاملPhenotypic and genotypic methods for typing Campylobacter jejuni and Campylobacter coli in poultry.
Human campylobacteriosis, an infection caused by the bacterium Campylobacter, is a major issue in the United States food system, especially for poultry products. According to the Center for Disease Control, campylobacterosis is estimated to affect over 2.4 million people annually. Campylobacter jejuni and Campylobacter coli are 2 species responsible for the majority of campylobacterosis infecti...
متن کاملDistribution and polymorphism of the flagellin genes from isolates of Campylobacter coli and Campylobacter jejuni.
The complex flagellar filaments of the LIO8 serogroup member Campylobacter coli VC167 are composed of two highly related subunit proteins encoded by the flaA and flaB genes which share 92% identity. Using oligonucleotide primers based on the known DNA sequence of both the flaA and flaB genes from C. coli VC167 in the polymerase chain reaction, we have shown conservation of both fla genes among ...
متن کاملComparison of molecular typing methods useful for detecting clusters of Campylobacter jejuni and C. coli isolates through routine surveillance.
Campylobacter spp. may be responsible for unreported outbreaks of food-borne disease. The detection of these outbreaks is made more difficult by the fact that appropriate methods for detecting clusters of Campylobacter have not been well defined. We have compared the characteristics of five molecular typing methods on Campylobacter jejuni and C. coli isolates obtained from human and nonhuman so...
متن کاملPrevalence of Campylobacter jejuni and Campylobacter coli in captive wildlife species of India
Campylobacteriosis is an important zoonotic disease and the prevalence of Campylobacter is largely unknown in the wildlife of India. A total of 370 samples, comprising of 314 fresh faecal samples from apparently healthy captive wild animals and birds, 30 stool swabs from animal care takers and 26 samples of the animals’ food and water were collected from G. B. Pant High Altitude Zoo, Nainital, ...
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ژورنال
عنوان ژورنال: Journal of Applied Microbiology
سال: 2003
ISSN: 1364-5072,1365-2672
DOI: 10.1046/j.1365-2672.2003.02101.x